Disulfide Oxidoreductase, Recombinant, E. coli (dsbA)
Product Sizes
500ug
£819.00
D3778-01-500UG
About this Product
- SKU:
- D3778-01
- Extra Details:
- Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids). Recombinant protein corresponding to DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight: ~21.1kD (189 amino acids) Amino Acid Sequence: AQYEDGKQYT/ TLEKPVAGAP/ QVLEFFSFFC/ PHCYQFEEVL/ HISDNVKKKL/ PEGVKMTKYH/ VNFMGGDLGK/ DLTQAWAVAM/ ALGVEDKVTV/ PLFEGVQKTQ/ TIRSASDIRD/ VFINAGIKGE/ EYDAAWNSFV/ VKSLVAQQEK/ AAADVQLRGV/ PAMFVNGKYQ/ LNPQGMDTSN/ MDVFVQQYAD/ TVKYLSEKK Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 21.1
- Physical State:
- Supplied as a liquid in 20mM Tris pH 7.5, 2mM EDTA
- Purity:
- ≥95% by SDS PAGE
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
