Deoxyribonuclease I (DNase I)
Product Sizes
100ug
£914.00
D3200-06-100UG
About this Product
- SKU:
- D3200-06
- Application:
- Immunoprecipitation, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications have not been tested. Recommended Dilutions: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:5000 Immunoprecipitation: 1:100 Optimal dilutions to be detemined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Bovine pancreatic Deoxyribonuclease I
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile dH2O.
- Purity:
- Purified by Ion Exchange chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes 31kD bovine Deoxyribonuclease I. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum and well as purified and partially purified deoxyribonuclease I, (bovine pancreas).
- Source:
- bovine
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
