Datura stramonium (DSL)
Product Sizes
5mg
£614.00
D1100-01A-5MG
About this Product
- SKU:
- D1100-01A
- Extra Details:
- The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to Nacetyllactosamine and oligomers containing repeating -acetyllactosamine sequences. Abranched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Sugar Specificity: [GlcNAc]1-3, N-Acetylglucosamine Inhibiting/Eluting Sugar: Chitin Hydrolysate Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Physical State:
- Supplied as a lyophilized powder. Reconstitute with 10mM HEPES buffered saline, pH 8.5, 0.1mM CaCl2. Do not vortex.
- Purity:
- Homogeneous by SDS-PAGE
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Lectins
