Cytokeratin 19
Product Sizes
50ug
£832.00
C9097-28-50UG
About this Product
- SKU:
- C9097-28
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 5F230 (Ks19.2 (Z105.6))
- Clonality:
- Monoclonal
- Extra Details:
- Tumors Specifically Detected: All tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma and thymoma. Represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:10 with PBS, pH 7.4. Incubate for 1 hr at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Cytokeratin 19 from MCF-7 cells. Epitope: aa352-368 (VRADSERQNQEYQRLMD) of the a-helical fragment.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Cytokeratin 19 at ~40kD on human glandular epithelia. Species Crossreactivity: rat, fish, bovine, rabbit. Does not react with mouse, woodchuck, chicken or Xenopus. Reactive with cultured cell lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29 and SSC-12. Detected Cytokeratin 19 in cholangio carcinoma of liver, renal cell carcinoma, transitional cell carcinoma of the bladder, ovar
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
