Cytokeratin 13
Product Sizes
50ug
£800.00
C9097-12C-50UG
About this Product
- SKU:
- C9097-12C
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- translate.label.attr.clone:
- 5F225
- Clonality:
- Monoclonal
- Extra Details:
- Represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen, paraffin-embedded tissue and cytological): 1:10, Protease pretreatment improves the performance with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Cytokeratin 13 of Mr 54,000 purified from human esophagus.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Human Cytokeratin 13. Species Crossreactivity: bovine and rat. Reactivities on Cultured Cell lines: Squamous cell CA (A-431 from epidermoid CA of vulva; RT 112, RT-4 of urinary bladder). Polypeptide reacting: Mr 54 000 human cytokeratin 13 with minor affinity to K14, Mr 50,000,K16, Mr 48,000, and K25. Tumors detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
