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Antibodies

Cytochrome P450 2B1

Product Sizes
100ul
£570.00
C9095-15B5-100UL
About this Product
SKU:
C9095-15B5
Application:
Western Blot
translate.label.attr.clone:
9L780
Clonality:
Monoclonal
Extra Details:
The Cytochrome P450 superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 will yield a strong band with 10ug of rat microsomes using Alkaline Phosphatase Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Mouse
Isotype:
IgG
Physical State:
Supplied as a liquid.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes rat Cytochrome P450 (2B1).
Source:
rat
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody