Cyclin E (FITC)
Product Sizes
100ul
£1055.00
C8455-21-FITC-100UL
About this Product
- SKU:
- C8455-21-FITC
- Application:
- Immunoprecipitation, Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 3G116
- Clonality:
- Monoclonal
- Extra Details:
- Cyclin E is a cell cycle protein, 50kD, that complexes with cdk2 and is required for DNA synthesis. Cyclin E/cdk2 activity peaks just before S phase. Cyclin E expression is mediated by E2F and is cleared by the ubiquitin proteolytic pathway after phosphorylation. Alterations in cyclin E expression in cancer has been correlated with increasing stage and grade of tumor. These results suggest that cyclin E may be useful as a prognostic marker for breast cancer and gastric cancer, and non-small cell lung cancer. Applications: Suitable for use in Western Blot, Immunoprecipitation and Gel Mobility Shift Assay. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Full-length recombinant protein corresponding to human cyclin E.
- Isotype:
- IgG2b,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human cyclin E. The epitope is located within the last 80aa of the C-terminal. Detects a ~50kD band on human HeLa cell lysates by Western Blot analysis.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
