Contactin 6 (Neural recognition molecule NB-3, hNB-3, Contactin-6 precursor, MGC133256, NB3, NB-3)
Product Sizes
100ug
£876.00
C7865-25C-100UG
About this Product
- SKU:
- C7865-25C
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- Contactin-6, is a 130kD member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1,2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5). Mouse Contactin-6 shares 90-96aa sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3,5-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6,8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5,7). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa20-998 from mouse Contactin-6 expressed in CHO cells (Q9JMB8).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Contactin-6. Species sequence homology: Recombinant mouse Contactin-3, Contactin-4, recombinant human Contactin-5, and TAG-1 (5%).
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
