Connexin 37 (CX37, Gap Junction Alpha 4 Protein, CXA-4) (Not for Export EU)
Product Sizes
100ul
£704.00
C7855-35-100UL
About this Product
- SKU:
- C7855-35
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Dalton. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane domains, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:100K using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- A 16 amino acid peptide sequence within the C-terminal cytoplasmic domain of mouse Cx37 (2) was coupled to KLH (carrier protein) via the N-terminal Cysteine
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Mouse immunogenic peptide sequence is specific for Cx37. No significant homology is seen with other Connexin. Species Sequence Homology: 93% and 87% sequence homology with the rat and human Cx37, respectively (3). The mouse peptide sequence also shows substantial homology (68%) with Xenopus Cx41 protein.
- Source:
- mouse
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Type:
- Antibodies:Polyclonal Antibody
