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Antibodies

Complement C5, Human

Product Sizes
50ug
£800.00
C7850-23-50UG
About this Product
SKU:
C7850-23
Application:
Western Blot, Immunohistochemistry
translate.label.attr.clone:
5F207
Clonality:
Monoclonal
Extra Details:
C5 component of human complement is a plasma protein consisting of two polypeptide chains, a, b, bound by disulphide bonds. It has a molecular weight of 190000. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70mg/ml. Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:10; Incubation Time 1hr at RT. Optimal dilution to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Mouse
Immunogen:
Human complement component C5.
Isotype:
IgG1
Physical State:
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity:
Purified by Protein A affinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human native C5 as well as with C5b. Allows detection of native complement component C5 and discrimination from activated C5b-9 complexes, especially in different forms of glomerulonephritis.
Source:
human
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody