Complement C3 (C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, C3, CPAMD1) (Not for Export EU)
Product Sizes
100ul
£704.00
C7850-12M-100UL
About this Product
- SKU:
- C7850-12M
- Clonality:
- Polyclonal
- Extra Details:
- Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2. Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin. C3 is an acute phase reactant. Synthesis of C3, a glycoprotein, is induced during acute inflammation. The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages. A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1663aa. This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds. Pro-C3 contains a signal peptide of 22aa residues, the beta chain (645 residues) and the alpha chain (992 residues). The 2 chains are joined by 4 arginine residues that are not present in the mature protein. Human C3 has 79% identity to mouse C3 at the nucleotide level and 77% at the amino acid level. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 (ECL) ELISA: 1:10,000-50,000 (50-100ng control antigen/well) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Highly purified human serum C3 protein.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human C3 protein.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
