Collagen Type XXV, alpha 1 (COL25A1, CLAC)
Product Sizes
100ug
£876.00
C7510-98E-100UG
About this Product
- SKU:
- C7510-98E
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65-75kD member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family. It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1-33) and a 600 aa extracellular domain (ECD) (aa 55-654). The ECD contains seven Collagen-like domains. Furin cleavage between Arg112-Glu113 generates a 70kD soluble fragment that homotrimerizes and binds to early ABeta amyloid deposits. There are at least five multiple splice forms that impact the ECD. Amino acids 139-146 and the seventh Collagen-like domain (aa 571-630) are most often affected. Over aa 189-654, human COL25A1 is 95% aa identical to mouse COL25A1. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhCOL25A1 is ~ 0.5ng/well. Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- NS0-derived rhCOL25A1 isoform 1. (aa 189 - 654)
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human COL25A1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
