Collagen Type II
Product Sizes
100ug
£540.00
C7510-21C-100UG
About this Product
- SKU:
- C7510-21C
- Application:
- Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:50,000 Immunohistochemistry: 1:50-1:400. Antigen retrieval with sodium citrate buffer, pH 6.0 is recommended. Immunoprecipitation: 1:100 Western Blot: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Collagen Type II from human knee cartilage and bovine nasal cartilage.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Collagen Type II. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Recognizes most mammalian Type II collagens and has ex
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
