Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5)
Product Sizes
100ug
£832.00
C5067-91B-100UG
About this Product
- SKU:
- C5067-91B
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins. Depending upon its expression profile, it may be a glycoprotein of 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons. Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein. It possesses a 393 aa extracellular region (aa31-423) that contains a Ser117 CS attachment site and an EGF-like domain (aa 371-413). There are at least two isoform variants. One shows a deletion of aa 487-513, while a second shows an alternaive start site at Met139 with the same deletion. Phosphorylation likely occurs on Ser249, and proteolysis generates a >75kD soluble fragment. Over aa 31-420, human NGC shares 84% aa identity with mouse NGC. Applications: Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other Applications not tested. Recommended Dilution: Western Blot: 1ug/ml SH-SY5Y human neuroblastoma cell Immunocytochemistry: 1-15ug/ml Perfusion frozen sections of mouse brain (cerebellum) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Val31-Gln420 of human Neuroglycan C extracellular domain, expressed in CHO cells.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Neuroglycan C. Shows approximately 50% cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
