CHD1 (CHD1, CHD-1, Chromodomain helicase DNA binding protein 1)
Product Sizes
100ug
£761.00
C3875-10E1-100UG
About this Product
- SKU:
- C3875-10E1
- Application:
- Western Blot
- translate.label.attr.clone:
- 11n46
- Clonality:
- Monoclonal
- Extra Details:
- CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205kD member of the SNF2/RAD54 helicase family of proteins. It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710aa in length. It contains two Ser-rich regions (aa1-70 and 117-137), two Chromo (chromatin-organizer-modifer) domains (aa272-364 and 389-452), a SNF2 family N-terminal domain (aa484-763) and a C-terminal helicase domain (aa792-943). There are at least 30 Ser/Thr phosphorylation sites. Over aa1531-1710, human CHD1 shares 92aa identity with mouse CHD1. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to Lys1531-Thr1710 from human CHD1 expressed in E. coli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CHD1. No cross-reactivity with recombinant human CHD1L, 5, or 7.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
