CD119 (CD119 Antigen, CDw119, FLJ45734, Immune Interferon Receptor 1, Interferon gamma Receptor alpha Chain, Interferon gamma Receptor 1, IFN-gamma Receptor 1, IFN gamma R1, IFN-gamma-R1, IFNGR 1, IFNGR1, IFNGR) (Biotin)
Product Sizes
50ug
£991.00
C2453-01G1-50UG
About this Product
- SKU:
- C2453-01G1
- Clonality:
- Polyclonal
- Extra Details:
- The high-affinity IFN-G receptor complex is made up of two type I membrane proteins, IFN-G R1 (IFN-G Ra) and IFN-G R2 (IFN-G Rb). Both proteins are members of the type II cytokine receptor family and share ~52% overall sequence identity. IFN-G R1 is the ligand-binding subunit that is necessary and sufficient for IFN-G binding and receptor internalization. IFN-G R2 is required for IFN-G signaling but does not bind IFN-G by itself. Human IFN-G R1 cDNA encodes a 499aa residue protein with a 17aa signal peptide, a 228aa extracellular domain, a 23aa transmembrane domain, and a 221aa intracellular domain. Human and mouse IFN-G R1 share 52aa sequence similarity and bind IFN-G in a species-specific manner. IFN-G R1 is constitutively expressed in most cell types. Soluble IFN-G R1 that binds IFN-G has been detected in biological fluids. The recombinant soluble IFN-G R1 has been shown to bind IFN-G with high affinity and is a potent IFN-G antagonist. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.1-0.4ug/ml ELISA (capture): 2-8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to aa26-253 from mouse CD119 expressed in NSO cells (Q91Y85).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, BSA. Reconstitute with sterile TBS, pH7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA to 200ug/ml. Labeled with Biotin.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse CD119.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
