CD103 (CD103 Antigen, HML-1 Antigen, Human Mucosal Lymphocyte Antigen 1, Huminae, Integrin alpha E, ITGAE, Integrin alpha IEL, MGC141996, Mucosal Lymphocyte 1 Antigen, Mucosal Lymphocyte Antigen 1) (FITC)
Product Sizes
500ug
£607.00
C2445-65D-500UG
About this Product
- SKU:
- C2445-65D
- Application:
- Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 2E7
- Clonality:
- Monoclonal
- Extra Details:
- CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. This antibody is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. The property of costimulation is not unique to the mouse since the combination of anti-human alphaIEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Hamster
- Immunogen:
- C57BL/6J mouse intestinal intraepithelial lymphocytes
- Isotype:
- IgG2
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse CD103 (aIELb7 integrin complex).
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
