CD97 (Leukocyte Antigen CD97, Seven Transmembrane Heterodimeric Receptor Associated with Inflammation, TM7LN1)
Product Sizes
100ug
£812.00
C2443-60B-100UG
About this Product
- SKU:
- C2443-60B
- Application:
- Flow Cytometry, Western Blot
- translate.label.attr.clone:
- 7H172
- Clonality:
- Monoclonal
- Extra Details:
- CD97 is a 116kD member of the EGF subfamily of LNB-7TM multispan receptors. It is expressed on smooth muscle and immune cells and is upregulated during inflammation. CD97 binds CD55 and chondroitin sulfate. The N-terminal extracellular domain contains five tandem EGF-like domains and one GPS domain. Alternate splicing generates two additional isoforms with deletions in the EGF-like region. A noncovalently-linked heterodimer composed of alpha (ECD) and beta (7TM) subunits is generated by proteolytic cleavage within the GPS domain. Within the region included in this immunogen, human CD97 shares 49%-53% aa identity with canine, mouse, and rat CD97. Applications: Suitable for use in Neutralization, Western Blot, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Flow Cytometry: 25ug/ml ELISA: 0.5-1ug/ml Neutralization: 0.5-2.5ug/mL in the presence of 4ug/mL Recombinant Human CD97. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- NS0-derived recombinant human CD97 (aa21-398) UniProt # P48960
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CD97.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
