CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7) (PE/CY5)
Product Sizes
100ug
£486.00
C2432-14E-100UG
About this Product
- SKU:
- C2432-14E
- Application:
- Immunoprecipitation, Flow Cytometry
- translate.label.attr.clone:
- 1G10
- Clonality:
- Monoclonal
- Extra Details:
- CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. 1-4 The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex.5, 6 This antibody locks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. 2, 7 Applications: • Flow cytometry 2, 7 • Immunoprecipitation 7 • In vivo and in vitro blocking of ligand binding 7 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Rat
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse CD80 (B7-1), Mr 45-47kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
