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Antibodies

CD79b (CD79b Antigen, CD79b Molecule Immunoglobulin-associated beta, CD79b Protein, B Cell Antigen Receptor Complex-associated Protein beta Chain, B Cell-specific Glycoprotein B29, B29, Ig-beta, IGB, Immunoglobulin-associated B29 Protein)

Product Sizes
100ug
£876.00
C2431-06L1-100UG
About this Product
SKU:
C2431-06L1
Application:
Flow Cytometry, Western Blot
Clonality:
Polyclonal
Extra Details:
CD79B is a 36-40kD member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with 44-49kD CD79A. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. Mature human CD79B is a 201aa type I tranamembrane glycoprotein (aa29-229). It contains an extracellular region with one V-type Ig-like domain (aa38-138) and an ITAM-containing cytoplasmic domain (aa181-229). There is an alternative splice form that shows a deletion of aa41-144 and appears after B cell activation. Human CD79A and B share only 26aa identity. Over aa29-159, human CD79B shares 54aa identity with mouse CD79B. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Sheep
Immunogen:
Recombinant protein corresponding to aa29-159 from human CD79B expressed in CHO cells (P40259).
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity:
Purified by immunoaffinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human CD79B. Species sequence homology: mouse CD29B (50%).
Source:
human
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody