CD49e (Integrin alpha5) (Not for Export EU)
Product Sizes
100ul
£825.00
C2405-02D-100UL
About this Product
- SKU:
- C2405-02D
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- CD49e is a type I membrane protein that associates non-covalently with Integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500-1:2000 Western Blot: 1:1000 detects integrin alpha 5 on 10ug of HUVEC lysates. HUVEC lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with C2405-02D (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: Use 5ul antibody for 5x10e6 cells. Immunohistochemistry (Acetone fixed tissues): 1:1000 Immunocytochemistry: 1:1000 Optimal dilutions to be determined by the researcher. Control: Mouse 3T3 fibroblasts, skin (basement membrane) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha 5 Integrin subunit.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Integrin alpha 5 at ~114kD. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 4, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and is therefore, intracellular. Species Crossreactivity: chicken, mouse, rat and hamster.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
