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Antibodies

CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE/Cy5.5)

Product Sizes
100ug
£475.00
C2400-05G-100UG
About this Product
SKU:
C2400-05G
Application:
Immunoprecipitation, Flow Cytometry, Immunohistochemistry
translate.label.attr.clone:
13/2.3
Clonality:
Monoclonal
Extra Details:
CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes.1-4 CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.4 Applications: • Flow cytometry 2, 3 • Immunoprecipitation 1, 3 • Radiometric quantitation of CD45 molecules on cell surfaces 1 • Immunohistochemistry (frozen sections) Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Rat
Isotype:
IgG2b
Physical State:
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy5.5.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms). Recognizes a framework epitope present on all CD45 isoforms.
Source:
mouse
Storage Conditions:
4[o]C Do Not Freeze
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody