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Antibodies

CD31 (PECAM-1, Platelet gpIIa Molecule)

Product Sizes
100ug
£953.00
C2383-22C-100UG
About this Product
SKU:
C2383-22C
Application:
Flow Cytometry, Immunohistochemistry, Western Blot
Clonality:
Polyclonal
Extra Details:
Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml Flow Cytometry: This antibody was tested in flow cytometry using mouse splenocytes. Dilute this antibody to 50ug/ml and add 10 μL of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Immunohistochemistry: 5-15ug/mL Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Goat
Immunogen:
NS0-derived rmCD31 extracellular domain
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity:
Purified by immunoaffinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Selected for its ability to recognize mouse CD31 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~10% crossreactivity with rhCD31 and rpCD31.
Source:
mouse
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody