CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (PE/CY5)
Product Sizes
100ug
£453.00
C2380-18F-100UG
About this Product
- SKU:
- C2380-18F
- Application:
- Immunoprecipitation, Flow Cytometry
- translate.label.attr.clone:
- 37.51
- Clonality:
- Monoclonal
- Extra Details:
- CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes, and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. 1-4 CD28 is a ligand for CD80/B7-1 and CD86/B7-2 on B cells and other antigen presenting cells, and plays an important role in the interaction between T cells and B cells. CD28 is a co-stimulatory receptor involved in many, but not all, T-cell independent immune responses.5-8 Applications: • Flow cytometry 2-5 • Immunoprecipitation 2 • In vitro costimulation of T cells and NK cells 1, 4, 7 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Hamster
- Isotype:
- IgG,l
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes CD28 costimulatory receptor, Mr 40kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
