CD25 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (PE/CY5)
Product Sizes
100ug
£435.00
C2275-82E-100UG
About this Product
- SKU:
- C2275-82E
- Application:
- Immunoprecipitation, Flow Cytometry
- translate.label.attr.clone:
- 3C7
- Clonality:
- Monoclonal
- Extra Details:
- The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.5 It is expressed at a higher level on CD4+CD8+ thymocytes.5, 6 It is also expressed on cultured epidermal Langerhans cells.7 The biochemical features of murine CD25 have been characterized in detail.8-10. This antibody, used in combination with C2275-89A in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. 2, 13 Applications: • Identification and enumeration of CD25+ cells by flow cytometry 1-4 • Immunoprecipitation 1, 2, 8, 10 • Inhibition of IL-2 or mitogen-induced T cell proliferation 13 • In vitro blocking of IL-2 binding 2, 12 Recommended Dilution: Flow Cytometry: ≤ 0.3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Rat
- Isotype:
- IgG2b,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the low affinity alpha chain IL-2 receptor, Mr 55kD (IL-2Ra).
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
