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Antibodies

CD24 (GPI-linked surface Mucin, Nectadrin) (Azide Free)

Product Sizes
500ug
£607.00
C2275-11F-500UG
About this Product
SKU:
C2275-11F
Application:
Immunoprecipitation, Flow Cytometry
translate.label.attr.clone:
30-F1
Clonality:
Monoclonal
Extra Details:
CD24 is expressed in B cells until their terminal differentiation into plasma cells.1 It has been used widely as a marker to fractionate different B-lineage differentiation stages.10 CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule.2-4 The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse.1, 3 The molecular mass is variable, ranging from 38-70kD.3 CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins.1-4 Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion.3 This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti-CD24 monoclonal antibody.6 Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.7 Applications: • Flow cytometry and cell sorting 3, 4, 7-10 • Immunoprecipitation 3 Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Rat
Immunogen:
Mouse thymus or spleen
Isotype:
IgG2c,k
Physical State:
Supplied as a liquid in PBS. No preservative added.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse CD24.
Source:
mouse
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody