CD24 (GPI-linked surface Mucin, Nectadrin, Heat stable antigen, HSA) (PE/CY5)
Product Sizes
100ug
£435.00
C2275-05E-100UG
About this Product
- SKU:
- C2275-05E
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 5K10 (91)
- Clonality:
- Monoclonal
- Extra Details:
- CD24, also known as heat stable antigen, is a phosphatidylinositol-linked cell surface glycoprotein that is expressed at all stages of B cell development and on most thymocytes.1-3 The absence of expression from mature T cells is closely associated with their maturation from CD4+CD8+CD24+ thymocytes to either CD4+CD8-CD24- or CD4-CD8+CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes.4-6 Its ligand is P-selectin (CD62P).7 CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes.1-3 Can be used to inhibit B-cell differentiation. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.3ug/10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE/CY5 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Rat
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE)/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse CD24; Mr 95kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
