CD22 (PE/CY5)
Product Sizes
100ug
£435.00
C2272-88F-100UG
About this Product
- SKU:
- C2272-88F
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 2D6
- Clonality:
- Monoclonal
- Extra Details:
- CD22, also known as Lyb-8, is a heterodimeric transmembrane glycoprotein and a member of a structurally related group of immunoglobulin (Ig) superfamily domain-containing proteins called the sialoadhesin family. It is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface concomitant with IgD, and is found on most IgM+IgD+ mature B lymphocytes. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via cross-linking of surface Ig increases CD22 expression.1, 2 CD22 associates with the B cell receptor (BCR) complex and mediates intercellular adhesion.2-4 Its intracellular domain is phosphorylated after antigen receptor crosslinking and is involved in negative regulation of B-cell activation.6-9 Applications: • Flow cytometry 2 • Blocking of B cell homotypic adhesion 2 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Rat
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recongizes CD22 (Mr 150kD), a B lymphocyte adhesion molecule.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
