CD19 (B Lymphocyte Antigen CD19, B4, Differentiation Antigen CD19, Leu 12, Leu-12, MGC12802) (PE/Cy5.5)
Product Sizes
100ug
£422.00
C2269-50R-100UG
About this Product
- SKU:
- C2269-50R
- Application:
- Immunoprecipitation, Flow Cytometry
- translate.label.attr.clone:
- 6D5
- Clonality:
- Monoclonal
- Extra Details:
- CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages.1-7 Terminally differentiated plasma cells do not express CD19.1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation.3, 4 All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites.1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow.7 Monoclonal antibody 3H1069 recognizes the same epitope as the published clone, 1D3 (1). Applications: • Flow cytometry 1-3 • Immunoprecipitation 1 • In vivo and in vitro functional studies 1, 2 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Mouse CD19-expressing K562 human erythroleukemia cells
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy5.5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes CD19 B-cell differentiation antigen, Mr 95kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
