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Antibodies

CD16 (PE/CY5)

Product Sizes
100Tests
£453.00
C2267-29H-100TESTS
About this Product
SKU:
C2267-29H
Application:
Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Western Blot
translate.label.attr.clone:
GRM1
Clonality:
Monoclonal
Extra Details:
CD16, a member of the immunoglobulin superfamily, is a 50-65kD glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction.1-5 Applications: • Flow cytometry • Immunohistochemistry (frozen sections) • Western blotting • Immunoprecipitation Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
Host:
Mouse
Isotype:
IgG2a
Physical State:
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human CD16.
Source:
human
Storage Conditions:
4[o]C Do Not Freeze
Type:
Antibodies:Monoclonal Antibody