CD8 (Leu2 T Lymphocyte Antigen, MAL, OKT8 T Cell Antigen, p32, T Cell Antigen Leu2, T Cell Surface Glycoprotein CD8 alpha, Suppressor/Cytotoxic T Cell) (PerCP-Cy5.5)
Product Sizes
50Tests
£832.00
C2259-36A-50TESTS
About this Product
- SKU:
- C2259-36A
- translate.label.attr.clone:
- 11C95
- Clonality:
- Monoclonal
- Extra Details:
- Comparative structural and functional studies of mouse and human T-cell antigens showed that human cytotoxic-suppressor T cells have a molecule homologous to the mouse Lyt-2,Lyt-3 molecule. The human homolog of Lyt-2,Lyt-3 is termed LEU2. It is selectively expressed on a subset of T cells and in structure is a multimeric macromolecule composed of individual disulfide- bonded subunits. LEU2, or T8, is expressed by most T lymphocytes with cytotoxic or suppressor function. The molecule appears to be composed of multimers of a 32kD and a 45kD polypeptide in thymocytes and of a 32kD polypeptide in peripheral blood lymphocytes. LEU2 (like its proposed murine homolog Lyt-2) may play a role in target-cell recognition. It has been shown that through alternative splicing of mRNA, an exon encoding a transmembrane domain of CD8 is deleted. This gives rise to a 30kD molecule that is secreted and exists as a monomer. The splicing pattern in man differs from that found in the mouse CD8 gene. This mRNA is also alternatively spliced, but an exon encoding a cytoplasmic region is deleted, giving rise to a cell surface molecule that differs in its cytoplasmic tail from the protein encoded by the longer mRNA. Neither protein is secreted. This is an example of the different splicing patterns of 2 homologous mouse and human genes giving rise to different proteins. The CD8 antigen is present on the human suppressor/cytotoxic T- lymphocyte subset as well as on a subset of NK lymphocytes. The CD8 antigen is expressed on 19% to 48% of normal peripheral blood lymphocytes and 60% to 85% of normal thymocytes. The CD8+ T- and NK-lymphocyte subsets can be further subdivided into the following groups: CD16+ NK lymphocytes that can express the CD8 antigen in low density; CD57+ T lymphocytes that express high-density CD8 antigen; and CD8+ CD62L+ lymphocytes that collaborate with CD8+CD62L– lymphocytes to generate suppression of B-lymphocyte function. CD8 cross-reacts with lymphocytes of some nonhuman primate species. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 20ul/test (1X10e6 cells in 100ul). Optimal dilutions to be determined by the researcher. Applications: IgG1, heavy, kappa light chains Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- CD8, clone Sk1, derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, gelatin, 0.005% sodium azide. Labeled with PerCP/Cy5.5.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CD8.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
