CD8a (Ly2, T cell Co-receptor, Leu2, T8) (PE/Cy5.5)
Product Sizes
100ug
£422.00
C2259-28J-100UG
About this Product
- SKU:
- C2259-28J
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 53-6.7
- Clonality:
- Monoclonal
- Extra Details:
- In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8alpha/Lyt-2) and a beta chain (CD8beta/Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8 Applications: • Flow cytometry 8, 9 • Immunohistochemistry (acetone-fixed frozen sections) 10 • Immunoprecipitation 8 • In vivo and in vitro depletion of CD8a+ cells 1, 4, 5 • Fractionation of CD8a+ T cells 10 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy5.5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the alpha chain of the murine CD8 heterodimer, Mr 32-34kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
