CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (PE/Cy7)
Product Sizes
100ug
£397.00
C2255-31J-100UG
About this Product
- SKU:
- C2255-31J
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- GK1.5
- Clonality:
- Monoclonal
- Extra Details:
- CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested (1,2). CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors (3-6). The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells (1,2). In vivo and in vitro treatment with the effectively depletes CD4+ cells (7,8). The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines (1,8-13). Applications: • Flow cytometry 1 • Immunohistochemistry (FS) 15 • Immunoprecipitation 1,2 • In vivo and in vitro depletion of CD4+ cells 6,7 • Inhibition of T helper function 1,2,8-13 • Fractionation of CD4+ cells 15 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Cloned mouse CTL line V41
- Isotype:
- IgG2b,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy7.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse CD4/L3T4, Mr 52kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
