CD4 (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (PE/CY5)
Product Sizes
100Tests
£492.00
C2255-11J-100TESTS
About this Product
- SKU:
- C2255-11J
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- RFT-4g
- Clonality:
- Monoclonal
- Extra Details:
- CD4 is a 59kD type I transmembrane glycoprotein and a member of the Ig-SF of cell surface receptors. It is expressed predominantly on the “helper/inducer” subpopulation of mature T lymphocytes, and on monocytes and macrophages. Domains 1 and 2 bind to MHC Class II antigens, while domains 3 and 4 may be involved in cis interactions with the CD3/TCR complex. CD4 functions as an accessary molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.1-6 Applications: • Flow cytometry • Immunohistochemistry (frozen sections) • Immunoprecipitation Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Mouse
- Immunogen:
- Thymocytes and E-rosetted lymphocytes
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CD4.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
