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Antibodies

CD3 (PE/Cy5.5)

Product Sizes
100Tests
£486.00
C2254-56R-100TESTS
About this Product
SKU:
C2254-56R
Application:
Immunoprecipitation, Flow Cytometry, Immunohistochemistry
translate.label.attr.clone:
UCHT1
Clonality:
Monoclonal
Extra Details:
CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. Applications: • Flow cytometry • Immunohistochemistry (frozen sections) • Immunoprecipitation • In vitro activation Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Mouse
Immunogen:
Human thymocytes and peripheral blood lymphocytes from a patient with Sezary cell leukemia.
Isotype:
IgG1
Physical State:
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/Cy5.5.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes the 20kD epsilon chain of the CD3/T cell antigen receptor complex.
Source:
human
Storage Conditions:
4[o]C Do Not Freeze
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody