CD3, epsilon (Early T Cell Marker) (PE/CY5)
Product Sizes
100ug
£384.00
C2254-11R-100UG
About this Product
- SKU:
- C2254-11R
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- C363.29B (YCD3-1)
- Clonality:
- Monoclonal
- Extra Details:
- CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains (1,2). CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. Immobilized monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present (1,3,4). The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody (1). Applications: • Identification and enumeration of CD3+ cells by flow cytometry 1, 2 • Immunoprecipitation 1 • In vitro depletion of CD3+ cells 1 • In vitro activation of T cells (accessory cells must be provided) 1, 3, 4 • Immunohistochemistry 5 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE and Cy5 conjugates are sensitive to light.
- Host:
- Rat
- Immunogen:
- IL-4 producing Th2 cell lines including D10
- Isotype:
- IgG2c,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE) and Cy5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
