Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)
Product Sizes
200ul
£581.00
C2087-01-200UL
About this Product
- SKU:
- C2087-01
- Application:
- Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Western Blot
- translate.label.attr.clone:
- 3C119 (CPP32 4-1-18)
- Clonality:
- Monoclonal
- Extra Details:
- Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. Effector Caspase 3 cleaves numerous substrates to effect the morphological changes associated with apoptosis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and Immunoprecipitation Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000. A band is seen at ~32kD for the inactive form of Caspase 3 and ~17-22kD for the active form of Caspase 3 Immunohistochemistry: 1:200-1:500 Immunohistochemistry/Immunofluorescence: 2ug/ml Immunoprecipitation: 2ug/mg lysate Immunohistochemistry (Paraffin): 1:200-1:500 Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Positive Control: Human kidney 293 cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Full-length recombinant protein corresponding to human Caspase 3.
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as liquid in PBS, 0.02% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Caspase 3. Species Crossreactivity: rat and mouse.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
