Catenin, gamma (Plakoglobin)
Product Sizes
100ug
£856.00
C2069-60-100UG
About this Product
- SKU:
- C2069-60
- Application:
- Immunoprecipitation, Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 3G62
- Clonality:
- Monoclonal
- Extra Details:
- Plakoglobin (gamma-catenin) is an 83kD protein that localizes to the cytoplasmic face of both desmosomal and adherens junctions. (4) Plakoglobin was first demonstrated to associate with the desmosomal cadherins, desmoglein and desmocollin, and was subsequently shown to associate with the classical cadherins E- and N-cadherin. (3,4,7,8,9,15) Plakoglobin’s interaction with E-and N-cadherin lead to its identification as the ~80kD protein originally called gamma-catenin. (8) Recent evidence indicates that the classical and desmosomal cadherins actually interact with distinct domains of the plakoglobin protein. (15) Further, the desmosomal cadherins have been shown to regulate the accumulation of plakoglobin in the cell by decreasing its turnover rate. (7) Plakoglobin (g-catenin) is highly homologous to both b-catenin and the product of the Drosophila segment polarity gene armadillo. (8,9,10,11,12) Plakoglobin (g-catenin) and b-catenin contain 13 copies of a 42–44 amino acid motif first identified in the Armadillo protein and referred to as armadillo repeats. (2,10,12) These armadillo repeats not only mediate the interaction between b and g-catenin and the cadherin cytoplasmic domain, but are also responsible for interactions with other cellular proteins. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:250 Immunofluorescence: 1:250 Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells, A431 cells, WI-38 human fibroblasts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Fusion protein consisting of the maltose binding protein fused to full length human plakoglobin protein.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human plakoglobin at ~83kD. Does not appear to crossreact with the related b-catenin protein (~92kD). Species Crossreactivity: mouse
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
