Carnitine Palmitoyl Transferase-2 (CPT-2) (Not for Export EU)
Product Sizes
100ul
£704.00
C1385-50-100UL
About this Product
- SKU:
- C1385-50
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase. CPT-2 (mouse/rat/human 658-aa, ~74kD, chromosome 1p32, ~20% identity with CPT1) is a ubiquitous malonyl-CoA-insensitive transferase localized in the inner mitochondrial membrane. It catalyzes the re-synthesis of acyl-CoA from acyl-carnitines. CPT-2 deficiency leads to the most commonly inherited, lipid myopathy in adults characterized by exercise-induced pain, stiffness, and myoglobinuria. The aa sequences of the two isoforms are ~61% identical. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:100,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- A 16-aa peptide sequence mapping near the C-terminus of mouse CPT-2L was synthesized, coupled to KLH
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Carnitine Palmitoyl Transferase-2 (CPT-2). 93% conserved in rat and 86% in human CPT-2. No significant sequence homology is seen with CPT-1 or any other protein.
- Source:
- mouse
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
