Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, KSST)
Product Sizes
100ug
£748.00
C1103-12-100UG
About this Product
- SKU:
- C1103-12
- Application:
- Immunoprecipitation
- translate.label.attr.clone:
- 11D37
- Clonality:
- Monoclonal
- Extra Details:
- The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4 O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST1, also known as keratan sulfate Gal6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate (3). It also.has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation (4). Human CHST1 shares 94% amino acid sequence identity with mouse CHSTI. Applications: Suitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg24-Ser411 from human CHST1, expressed in CHO cells.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human CHST1. In direct ELISAs, ~25-100% cross-reactivity with recombinant mouse CHST1 and no cross-reactivity with recombinant human CHST2, 3, 4, 5, 6, 7, 10, and 14 is observed.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
