Calcitonin Receptor Like Receptor (CRLR) (Not for Export EU)
Product Sizes
100ul
£704.00
C0115-90-100UL
About this Product
- SKU:
- C0115-90
- Clonality:
- Polyclonal
- Extra Details:
- The calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175aa; rat 182aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 (ECL). ELISA: 1:10,000-1:50,000. Control peptide can be used to coat plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: C0115-80: Calcitonin Receptor Like Receptor, Human, Control Peptide (CRLR) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide consisting of 20aa mapping near the cytoplasmic, C-terminus of rat CRLR (KLH).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Calcitonin Receptor Like Receptor. No significant sequence homology is detected with other CRLR. Species sequence homology: Mouse: 90%; human and chimp: 70%; porcine: 64%.
- Source:
- rat
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
