Cadherin N (Cadherin 2, Neuronal, CDH2)
Product Sizes
100ug
£832.00
C0108-35M-100UG
About this Product
- SKU:
- C0108-35M
- Application:
- Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Western Blot
- translate.label.attr.clone:
- 3G49 (3B9)
- Clonality:
- Monoclonal
- Extra Details:
- Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins beta-catenin and gamma catenin, which both bind to the actin binding protein alpha catenin. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1-3ug/ml Immunoprecipitation: 3-5ug/IP reaction Immunohistochemistry (FFPE): 1-5ug/ml. Heat induced epitope retrieval required. Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to the intracellular domain of chicken N-cadherin.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes chicken N-cadherin. Species Crossreactivity: human, mouse, rat and porcine
- Source:
- chicken
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
