Cadherin N (Cadherin 2, Neuronal, CDH2) (MaxLight 550)
Product Sizes
100ul
£1088.00
C0108-35M-ML550-100UL
About this Product
- SKU:
- C0108-35M-ML550
- Application:
- Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Western Blot
- translate.label.attr.clone:
- 3G49 (3B9)
- Clonality:
- Monoclonal
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins beta-catenin and gamma catenin, which both bind to the actin binding protein alpha catenin. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin fixed, paraffin embedded tissues. Heat induced epitope retrieval required. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to the intracellular domain of chicken N-cadherin.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes chicken N-cadherin. Species Crossreactivity: human, mouse, rat and porcine
- Source:
- chicken
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
