Cadherin 11 (OB-Cadherin, Osteoblast, CDH11) (HRP)
Product Sizes
100ul
£1144.00
C0107-07-HRP-100UL
About this Product
- SKU:
- C0107-07-HRP
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 3G41 (5B2H5)
- Clonality:
- Monoclonal
- Extra Details:
- Cadherins are a large family of Ca(2+)-dependent cell-cell adhesion molecules. Classical cadherins contain five EC domains and a conserved cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Cadherin 11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme and a strong expression has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. A variant Cadherin 11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin 11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested, but not in non-invasive cell lines, suggesting that cadherin-11 may be a marker for aggressive, invasive tumor subsets. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml Immunoprecipitation: 5ug. Native, human; Not tested for mouse. Immunohistochemistry (Frozen): 5-10ug/ml. Incubate for 30-60 minutes at RT or overnight at 4°C. Optimal dilutions to be determined by the researcher. Positive Control: MDA.MB231 cells (human) and mouse L fibroblast lysates. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to an intracellular domain of human Cadherin 11.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the ~110kD human Cadherin 11 protein. Crossreactivity with other cadherin family members has not been observed. Species Crossreactivity: mouse
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
