Cadherin 11 (OB-Cadherin, Osteoblast, CDH11) (Biotin)
Product Sizes
100ul
£1144.00
C0107-07-BIOTIN-100UL
About this Product
- SKU:
- C0107-07-BIOTIN
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 3G41 (5B2H5)
- Clonality:
- Monoclonal
- Extra Details:
- Cadherins are a large family of Ca(2+)-dependent cell-cell adhesion molecules. Classical cadherins contain five EC domains and a conserved cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Cadherin 11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme and a strong expression has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. A variant Cadherin 11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin 11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested, but not in non-invasive cell lines, suggesting that cadherin-11 may be a marker for aggressive, invasive tumor subsets. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml Immunoprecipitation: 5ug. Native, human; Not tested for mouse. Immunohistochemistry (Frozen): 5-10ug/ml. Incubate for 30-60 minutes at RT or overnight at 4°C. Optimal dilutions to be determined by the researcher. Positive Control: MDA.MB231 cells (human) and mouse L fibroblast lysates. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to an intracellular domain of human Cadherin 11.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the ~110kD human Cadherin 11 protein. Crossreactivity with other cadherin family members has not been observed. Species Crossreactivity: mouse
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
