CABP9K (D9K, CALB3, CABP1) (Not for Export EU)
Product Sizes
100ul
£704.00
C0104-65-100UL
About this Product
- SKU:
- C0104-65
- Clonality:
- Polyclonal
- Extra Details:
- Calcium is the most abundant cation and is required for many physiological activities and acts as a second messenger in signal transduction. Only 1% of Ca is present in ionic form in biological fluids. Ca concentration is regulated by calcitropic hormones that act on bone, kidney and intestine. Extracellular Ca+- levels are sensed and regulated by Calcium Sensing receptor (CASR). When Ca levels are limiting then it must be taken up by active, transcellular pathways. Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the “trigger” and the “buffer” CaBPs. The conformation of “trigger” type CaBPs changes upon Ca2+ binding and exposes regions on proteins that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. CABP9K (mouse, rat and human 79aa; chromosome Xp; ~9kD) is a cytosolic Ca-binding protein initially found in rat pancreas. It is also expressed in intestine, placenta, uterus and kidney. Its expression is controlled by vitamin D and sex hormone in a tissue specific manner. In keeping with its role in Ca- transport, its expression is highest in duodenal villus enterocytes. It is further shown that CABP9K is only expressed in differentiated enterocytes. CABP9K gene also contains Cdx2-homeoprotein binding sites, and that Cdx2 may play a crucial role in CABP9K transcription. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 using 50-100ng C0104-60 Control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 16aa within the N-terminal Ca-binding domain of mouse CABP9K. Species Sequence Homology: rat; 82%, porcine; 75%, bovine; 62%, human; 56%
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse CABP9K at ~9kD. No significant sequence homology is seen with D28K or other CABPs.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
