c-Myc (Myc, myc-1, Cellular myelocytomatosis oncogene, Myc2, Niard, Nird, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog)
Product Sizes
100ug
£953.00
C0035-21A-100UG
About this Product
- SKU:
- C0035-21A
- Application:
- Western Blot, Immunohistochemistry, ChIP
- Clonality:
- Polyclonal
- Extra Details:
- Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max. c-Myc is often over-expressed and mutated in hematopoietic tumors. Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA. Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins. Applications: Western Blot: This antibody can be used at 2.0ug/ml with the appropriate secondary reagents to detect human c-Myc. Immunohistochemistry: This antibody will detect human c-Myc in cells and tissues. The working dilution is 5-15ug/ml. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human c-Myc. The detection limit for rhc-Myc is approximately 0.2 ng/well. ChIP- 5ug/5x10e6 cells Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- E. coli-derived rhc-Myc (aa66-201)
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Selected for its ability to recognize human c-Myc in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1% crossreactivity with rhL-Myc and rhN-Myc.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
