Cbl (CBL, CBL2, C-CBL, RNF55, Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence, Oncogene CBL2)
Product Sizes
100ug
£761.00
C0013-26K-100UG
About this Product
- SKU:
- C0013-26K
- Application:
- Western Blot
- translate.label.attr.clone:
- 11D16
- Clonality:
- Monoclonal
- Extra Details:
- CBL (Lake Casitas, California mouse B-lineage Lymphoma; also RING finger protein 55) is a 120 -125kD cytosolic member of the CBL family of proteins. It is widely expressed, being found in cells such as fibroblasts, adipocytes, osteoclasts, podocytes and mammary epithelium. CBL negatively regulates RTK signaling by acting as an E3 ubiquitin ligase. Following activation via phosphorylation, CBL will interact with multiple signaling molecules such as Src, ZAP-70, EGFR and SHP1. Human CBL is 906aa in length. It contains an N-terminal phosphoTyr-binding region that consists of one a-helix bundle, an EF hand segment, and an SH2 domain (aa47-342), a RING finger domain that interacts with E2 enzymes (aa381-423) a Pro-rich SH3 domain (aa477-688), and a Leu-zipper/Ubiquitin-associated region (aa86-892). There is one potential alternative start site at Met55. Over (aa775-906, human CBL shares 91% aa identity with mouse CBL. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml with Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp775-Thr906 from human CBL expressed in E. coli.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trelahose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human and mouse CBL.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
