Brain-Specific Angiogenesis Inhibitor 3 (BAI3)
Product Sizes
100ug
£812.00
B2704-12C-100UG
About this Product
- SKU:
- B2704-12C
- Application:
- Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 8H174
- Clonality:
- Monoclonal
- Extra Details:
- Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177kD, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296-508), and one GPS domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI3 is 98% aa identical to mouse BAI3. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml in Immersion fixed paraffin-embedded sections of human brain (cortex) Flow Cytometry: 0.25ug/10e6 cells in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- NS0-derived recombinant protein corresponding to Ala25-Thr880 from human BAI3.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human BAI3.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
