BLAME, Recombinant Human (B-lymphocyte activator macrophage expressed)
Product Sizes
50ug
£957.00
B2106-50UG
About this Product
- SKU:
- B2106
- Extra Details:
- Human BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses. Mature human BLAME consists of a 211aa ECD that contains two Ig V-like domains, a 21aa transmembrane segment, and a 31aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-γ stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes. Molecular Mass: The recombinant human BLAME is a monomeric protein. Based on N-terminal amino acid sequencing, the recombinant protein starts at Ala 23 and it has a calculated molecular mass of 24.7kD. As a result of glycosylation, the recombinant monomer migrates as an approximately 35-40kD protein in SDS-PAGE under reducing conditions. Endotoxin: ≤ 1.0 EU per 1ug of the protein as determined by the LAL method. Biological Activity: Measured by its ability to bind biotinylated rhBLAME in a functional ELISA type binding assay. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
- Molecular Weight:
- 24.7
- Physical State:
- Supplied as a lyophilized powder from 20 mM Citrate, 0.3 NaCl, pH 6.0. Reconstitute with 500ul sterile PBS.
- Purity:
- ≥ 90% as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1.0EU/1ug (LAL)
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
